Homa Hemmati

2 Questions 18 Answers 0 Followers

Questions related from Homa Hemmati

How can I solve the problem in cloning a PCR amplified gene into a vector?

I am trying to clone two genes on one single vector. One gene is 1800 bp and the other 2900 bp. My vector is 10 kb ( pBIN Vector) and doesn't have my desired restriction sites so I designed...

09 September 2014 3,364 21 View

Why did I get band for RNA after PCR?

I did cDNA synthesis after RNA extraction including a negative control containing RNA and water with no RT. I used all my samples for regular PCR While I included a negative control containing...

02 February 2014 3,725 15 View