Holt Messner

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Questions related from Holt Messner

Which method of euthanasia is most appropriate for preserving the redox state of proteins from whole organs?

I've sacrificed a small number of mice via isofluorane + CO2 and quickly took all major organs and flash froze them with liquid nitrogen. The goal is to preserve the redox state of proteins in...

19 September 2017 8,276 3 View

Can mitoSOX be used to probe mitochondria which have previously been fixed with formalin?

The literature I've read seems to indicate mitoSOX is only used with living cells. I'm interested in probing for superoxide anions within the mitochondria of several necropsied organ tissue types....

02 March 2017 3,796 3 View