Any suggestions on growing Ins-1e cells as this is a new cell line for me?

I've had 2 cryovials given to me and I can't seem to get the cells to attach & grow. Complete media is 10% FBS, RPMI 1640 w/ L-glut, Sodium Pyruv, HEPES, Pen/Strep, & BME.

08 August 2016 6,478 0 View