I am seeing cell death upon transiently transfecting CRISPR-dCas9 machinery into A549 cells with Lipofectamine 3000?

I am trying to transiently co-transfect dCas9, gRNAs, and a puromycin-resistance plasmid into A549 cells using Lipofectamine 3000, but I see almost complete cell death by 4 days post-transfection...

05 August 2021 466 3 View