In my first trial in Methylation specific PCR, i got non-specific bands considering the methylated and unmethylated control, Why this happened?

My PCR Protocol was: Hot start: 95 °C for 10 min Denaturation: 15 s at 94°C, Annealing for 30 s at 61°C, Extension step for 30 s at 72°C; Final extension for 10 min at 72°C; A hold at 6°C. For 35...

07 July 2015 302 3 View