Hoang Tam Nguyen-Thai

4 Questions 8 Answers 0 Followers

Questions related from Hoang Tam Nguyen-Thai

Do you know what happened in my real time PCR?

I use the amplicon to be a positive control for a multiplex taqman realtime PCR assay. So I designed the amplicon and checked the sequence of amplicon, its sequence is OK for the primers and...

06 June 2015 779 6 View

Which statistical methods are used to analyse data of mRNA expression?

I'd like to have statistics for my genes expression data, which is the 2-deltaCT values from qRT-PCR data. I have 20 genes, their expressions were surveyed by time points. I used two-way ANOVA...

01 January 2014 2,159 2 View

How to identify primary normal cells?

I established primary normal cell cultures from human foreskin. Then identifying these cells by ICC method with anti-Vimentin and anti-Cytokeratin19 antibodies. The cell showed positive signal to...

09 September 2013 1,489 6 View

The appearance of huge cells in autophagic cells is normal or not?

I'm setting up AO staining to detect autophagy. The first observation, Tamoxifen-induced autophagy on MCF-7 cells had many huge cells (figure attached). Is that normal in autophagic cells or not?

01 January 2013 1,360 6 View