3 Questions 5 Answers 0 Followers
Questions related from Hoang Huu Nguyen
Well, In my parent's farm, there are quite interesting plants (Acacia mangium species) whose top of leaf attached with some leaves of other (Mimosa pudica). I mean that the leaves of Mimosa pudica...
08 August 2017 5,135 4 View
Should I clone a gene with RBS found in upstream of the gene into pET28a(+) for expression in E. coli BL21(DE3) or I just need to clone only gene sequence started from ATG ??? Thank you for your help!
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I wonder lacking RBS in the upstream of ErmE gene but ErmE is controlled effectually under ErmE promoter. Could you explain this clearly?
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