Hitesh Gelli Praveenkumar

4 Questions 4 Answers 0 Followers

Questions related from Hitesh Gelli Praveenkumar

How to prevent high TEER values while differentiating Caco2 cells on permeable supports?

I am trying to establish intestinal absorption/permeability model using Caco2 cell line. I seed Caco2 cells on 12 well permeable filters and grow them for 21 days for them to differentiate. I...

21 September 2021 333 4 View

Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

15 October 2020 5,272 4 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

10 July 2020 3,243 5 View

For long term, is it better to store human peripheral blood derived monocytes or differentiated macrophages?

For my project, I am currently generating M1 and M2 macrophages from human PBMCs. For later use, is it better to store monocytes and differentiate them whenever I want macrophages, or directly...

08 January 2020 998 3 View