What causes the smear below the band in electrophoresis?

When the PCR product was electrophoresed, such a band was visible and smear was seen under the band. Cycle conditions were (1) 94.0°C for 3 min, (2) 94.0°C for 10 sec, (3) 55.0°C for 10 sec, (4)...

01 March 2023 335 7 View