Hirdesh Kumar

2 Questions 2 Answers 0 Followers

Questions related from Hirdesh Kumar

How to prepare cryoEM grid for highly negative charged protein?

My protein has a pI of 4.1 and I am purifying it in Hepes(10mM)NaCl (150mM) buffer. When I freeze my protein sample for cryoEM using vitrobot, I dont see any particle. I glow-discharge my grid...

26 July 2018 5,658 1 View

How to enchance protein oligomerization ?

Preliminary negative stained samples show that my protein forms tetramer but I also have lower order entities (monomer, dimer etc).. I need homogeneity before I proceed to next step.. How can I...

24 May 2018 1,478 3 View