What would be the best strategy to optimize the PCR conditions for the gene COI using the primer pair Chmf4 and Chmr4 in cyprinid fish in this case?

I carried out a PCR reaction for a group of cyprinids fish to amplify the gene mitochondrial cytochrome oxidase 1 subunit (COI) using the pair of primers Chmf4 (5' TYT CWA CWA AYC AYA AAG AYA TCG...

30 September 2016 5,716 3 View