Hind Hussein

2 Questions 4 Answers 0 Followers

Questions related from Hind Hussein

IgG depletion method from mouse serum to avoid competition in an antigen-specific IgE ELISA?

I'm trying to measure OVA-specific IgE with a DIY ELISA, with an OVA coating. The issue I am facing is that since in the model I am using OVA-specific IgGs are produced in much higher...

18 October 2023 6,983 1 View

Has anybody ever had trouble spinning down naive B cells ?

After doing a MACS sort and a FACS sort to isolate naive B cells, I've had troubles spinning down my KO cells. I first used the conditions mentioned in the protocol: 400g for 5 min, in 15 or 50ml...

08 June 2016 9,495 5 View