How to do kinetic assay with nitrocefin? Is there any particular buffer other than phosphate buffer?

Hello I am working with recombinant β-lactamase enzymes (350-400 aa) with C-terminal his tag and the tag is not cleaved. I know nitrocefin is a chromogenic cephalosporin for β-lactamase activity...

04 February 2020 1,550 3 View