Hesham Abdullah

3 Questions 6 Answers 0 Followers

Questions related from Hesham Abdullah

Can someone advise on how to ligate 2000 bp insert into 12.7 Kb vector?

I am working with a specific synthesized genes which comes ligated into PUC57 vector. The 3 genes are ligated separately into pUC57 cloning vector (2710 bp) and all ligated with EcoRI BamHI. The...

07 July 2013 937 14 View

Is there any probability to show a fragment size 2300 bp being increased to more than 3000 bp after ligation?

I am working with a specific synthesized genes which comes ligated with PUC57 vector with a gene size of 2300 bp. I need to clone this gene again into a plant expression vector which has a size...

05 May 2013 7,331 4 View

Anyone have 10-beta Competent E. coli (High Efficiency) for electroporation or heat shock transformation?

I used T4 DNA ligase to ligate an insert has size about 2000 base pair into a vector has size 12.7 Kilo bases. I do everything possible to get colonies before I have contacted Biolab inc. and they...

04 April 2013 6,042 1 View