Herman Foo

2 Questions 2 Answers 0 Followers

Questions related from Herman Foo

Why there are a lot of colonies but no target fragment inserted?

I am going to insert a 6.3kb fragment into a 5.3kb plasmid. I use SAM1 digestion to cut the blunt end on the plasmid which is CCCGGG. I designed a set of primers to amplify the target fragment...

13 November 2022 7,352 3 View

How do I select substitution of codon without altering original function of the protein?

I want to mutate the ATGs of a gene, but I also want to not influence the original function of the protein. May I know whether there are criteria for selecting substitution of codon?

08 September 2022 1,719 1 View