Henriette Andresen

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Questions related from Henriette Andresen

Less protein in samples when using 0.1 M HCl and 5% TCA for cell lysis with both Bradford and microBCA - interference? Scraping? Ideas?

Hello everybody! I always get so good answers and help, so I'll ask you experts again. I use rat cardiac fibroblasts and do ELISA to measure cGMP. For lysis, the protocol says one can use either...

31 May 2017 1,229 3 View

Dissociation agent for adherent cells that do not affect membrane receptors?

Hi! Does anyone have a tip for a dissociation agent that isn't Trypsin-EDTA or affect membrane receptors? Trypsin cleaves our membrane receptors. I use QBIHEK293A cells. They are like HEK293...

23 November 2016 5,430 4 View