Heng Zhang

3 Questions 12 Answers 0 Followers

Questions related from Heng Zhang

Has anyone purify protein bound to strep-tactin XT using desthiobiotin?

We just bought the new strep-tactin XT for protein purification. The manufacture recommends to use 50 mM biotin to elute the protein. While 2.5 mM desthiobiotin is suggested for protein...

06 June 2019 4,968 4 View

Why is my native protein expressed well in E coli but double mutant has bad expression?

Hi all, I am expressing a bovine protein in E coli dh5a. My native protein expressed very well in bacteria but my double mutant and triple mutant hardly have any expression. only a faint band can...

05 May 2015 1,751 13 View

What is the desired resolution of a protein crystal after heavy metal soaking?

Hi all, I am a newbie on crystallography. Currently I am working on a protein which we had one set of data around 2.5 angstrom. My supervisor suggested me to do heavy metal soak to solve the phase...

03 March 2015 9,826 7 View