Heng Zhang

2 Questions 9 Answers 0 Followers

Questions related from Heng Zhang

Has anyone purify protein bound to strep-tactin XT using desthiobiotin?

We just bought the new strep-tactin XT for protein purification. The manufacture recommends to use 50 mM biotin to elute the protein. While 2.5 mM desthiobiotin is suggested for protein...

14 June 2019 5,452 5 View

Why is my native protein expressed well in E coli but double mutant has bad expression?

Hi all, I am expressing a bovine protein in E coli dh5a. My native protein expressed very well in bacteria but my double mutant and triple mutant hardly have any expression. only a faint band can...

20 May 2015 2,112 12 View