Heng Zhang

4 Questions 11 Answers 0 Followers

Questions related from Heng Zhang

Has anyone purify protein bound to strep-tactin XT using desthiobiotin?

We just bought the new strep-tactin XT for protein purification. The manufacture recommends to use 50 mM biotin to elute the protein. While 2.5 mM desthiobiotin is suggested for protein...

14 June 2019 5,506 5 View

Heavy metal soaking dataset having high anomalous completeness but having no significant anomalous signal?

Hi everyone, I am trying to solve my crystal structure using SAD. And recently I just collected one heavy metal soaked crystal diffraction dataset. This dataset has a very high anomalous...

20 October 2015 6,186 0 View

Why is my native protein expressed well in E coli but double mutant has bad expression?

Hi all, I am expressing a bovine protein in E coli dh5a. My native protein expressed very well in bacteria but my double mutant and triple mutant hardly have any expression. only a faint band can...

20 May 2015 2,146 12 View

What is the desired resolution of a protein crystal after heavy metal soaking?

Hi all, I am a newbie on crystallography. Currently I am working on a protein which we had one set of data around 2.5 angstrom. My supervisor suggested me to do heavy metal soak to solve the phase...

30 March 2015 7,719 6 View