Hemanth Kumar

2 Questions 15 Answers 0 Followers

Questions related from Hemanth Kumar

How do I reduce the loading time of protein on chromatography columns?

I'm trying to reduce the loading time of desired protein from clarified harvest on affinity columns, but I'm not getting it. We should select the resin based on the binding capacity and also...

10 October 2014 4,727 10 View

How do you avoid formation of aggregates in proteins?

I am purifying therapeutic proteins (MAbs) from a mammalian origin. I have observed formation of aggregates during long term storage. To avoid this I am storing them at 20°C instead of 2-8°C. I am...

10 October 2014 4,910 14 View