Hemant Kardam

2 Questions 8 Answers 0 Followers

Questions related from Hemant Kardam

concentration of DNA for poly(A) tailing

Can anybody help me , what concentration of amplified DNA i should take for poly(A)tailing so that i could be able to insert my gene into pGEM-Teasy vector through TA cloning

11 November 2011 2,434 0 View

Cloning of 3kb gene into pGEM-T vector

I am trying to clone 3kb gene into pGEM-Teasy . To amplify the insert , i am using pfu , so i do poly(A) tailing. I am taking 135ng/ul of amplified gene to add poly(A) tailing. After that , i am...

11 November 2011 7,084 2 View