Hemant Joshi

3 Questions 7 Answers 0 Followers

Questions related from Hemant Joshi

What would be best reagent to achieve efficient transfection method for MKN-45 cells ? Thanks.

I have to Knock-out a gene using TALEN. So, its rather big plasmid.

07 July 2017 2,586 1 View

How can I culture mice Lung fibroblast cells?

I am using DMEM from gibco for the culturing, but cells seems not happy. I have also tried DMEM Glutamax but in all case cells do not survive more than one month. And the growth is very slow. Is...

08 August 2014 5,923 9 View

Could anybody suggest how to make MEFs transiently transfected by PEI method?

09 September 2013 9,579 1 View