Hemamali W.P.E.H.

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Can anyone please explain why his-tagged protein are tightly bound to the Ni-NTA agarose beads?

My protein of interest has 6His tag. When it is bound to Ni beads it cannot be eluted with imidazole. PI of my protein is 7.04 Lysis buffer pH is 7.4 I used up to 1M imidazole in the...

12 December 2018 2,306 3 View