Helga Szakadati

2 Questions 4 Answers 0 Followers

Questions related from Helga Szakadati

Gene editing with CRISPR/Cas9 in S.cerevisiae works with one strain, but not with another one (same gene, no alterations). Why?

I am trying to knock out a gene in 2 strains of S.cerevisiae, BY4743 and W303 with CRISPR/Cas9. I am using the same gRNA and donor DNA for both strains because I want to knock out the same gene...

08 October 2023 6,028 7 View

How much non-coding bases should I leave between the Whi5 protein (yeast) and GFP (primer design)?

So, I would like to tag the Whi5 protein of the Saccharomices Cerevisiae. I would like to do it by PCR: first I want to multiply the GFP-kanMX casette part of the pym27 plasmid with primers that...

26 March 2020 9,524 2 View