Why bone marrow cells arrest at DN3/DN4 stage of T cell differentiation when grown on OP9-DL1 cells?

I am using the OP9-DL1 co-culture system to differentiate mouse bone marrow cells into double positive T cells. I infected the bone marrow cells with a retrovirus, selected the GFP+ cells, and...

11 August 2017 6,694 1 View