Helen Reynolds

2 Questions 3 Answers 0 Followers

Questions related from Helen Reynolds

Anyone know a good basic protocol for a cell based ELISA as a starting point?

I have had poor results with a regular ELISA so I plan to use a cell line which highly expresses my target protein as my 'coating antigen' to see if I get better results. I have never attempted...

10 November 2021 5,618 1 View

EDTA or Heparin as the anti-coagulant for buffy coat collection for PBMC preparation?

For as long as we can remember we have requested lithium heparin be used as the anti-coagulant from our buffy coat suppliers. This was largely due to a former lab head who insisted this was the...

26 September 2019 3,958 4 View