4 Questions 17 Answers 0 Followers
Questions related from Hefin Jones
Hi all, I'm working with primary, human monocyte-derived macrophages and have been polarizing them in vitro with LPS+IFNy or IL-4 (or medium alone). I've done this many times before in human and...
03 March 2015 1,594 9 View
Hi all, I'm trying to calculate the theoretical time for a vesicle to sediment during centrifugation. I have (I think) all the information, but the numbers I'm getting out don't seem to make...
03 March 2015 1,355 4 View
Hi, We've been trying to perform Ca2+ flux experiments by dying cells with FURA-2, and then measuring fluorescence using a plate reader in a 96-well format. The protocol has worked beautifully...
03 March 2015 6,914 3 View
Hi, I'm used to culturing primary human monocytes to generate macrophages in vitro, but three donors-worth of cells in a single week have had to be thrown out. I isolate PBMC form whole blood...
02 February 2015 297 15 View