Hefin Jones

4 Questions 17 Answers 0 Followers

Questions related from Hefin Jones

Why would macrophages upregulate CD86 and MHCII in response to IL-4?

Hi all, I'm working with primary, human monocyte-derived macrophages and have been polarizing them in vitro with LPS+IFNy or IL-4 (or medium alone). I've done this many times before in human and...

03 March 2015 1,591 9 View

How can I calculate the sedimentation time of vesicles?

Hi all, I'm trying to calculate the theoretical time for a vesicle to sediment during centrifugation. I have (I think) all the information, but the numbers I'm getting out don't seem to make...

03 March 2015 1,353 4 View

Why would cells detach with FURA-2 staining?

Hi, We've been trying to perform Ca2+ flux experiments by dying cells with FURA-2, and then measuring fluorescence using a plate reader in a 96-well format. The protocol has worked beautifully...

03 March 2015 6,913 3 View

Why would primary human monocytes clump together to form a "stringy" aggregate in culture?

Hi, I'm used to culturing primary human monocytes to generate macrophages in vitro, but three donors-worth of cells in a single week have had to be thrown out. I isolate PBMC form whole blood...

02 February 2015 296 15 View