How I can know the knocking out effect of specific target sequence by the CRISPR/Cas9 using RT-PCR?

I am doing knocking out for specific sequence by pX458 plasmid and I already did qPCR after 72h of transfection, I have used pX458 plasmid without gRNA as negative control but it seems that has...

20 December 2018 5,795 2 View