He Huang

4 Questions 6 Answers 0 Followers

Questions related from He Huang

Why there was no knock-down of target gene in some lentiviral shRNA stably transduced cell clones ?

I have performed lentiviral shRNA stable-transduction. Transduced cells were selected with puromycin. After all, 10 cell clones was isolated. The targeted gene was knocked down in 4 of the 10...

10 October 2016 7,794 3 View

Can the inserted sequence affect the replication of lentiviral transfer vector in E.coli?

I am inserting the orf of EMSY gene into lentiviral transfer vector pLVX (the EMSY orf was purchased from origene. pLVX was used to generate several constructs sucessfully in our lab). I have...

04 April 2015 9,162 5 View

Does anyone have experiences with Immuno-Precipitation of ApoB?

ApoB is large protein, ~550KDa. I searched the literature and most of the published articles brifely described the prosedure of IP. They added SDS in lysis buffer and lyzed the cells overnight to...

11 November 2014 844 2 View

Where does lentiviral shRNA insert into the genome?

Can the insertion of shRNA into host cell genome influence certain gene expression? How can I know the inserted site of lentiviral shRNA in a stably transduced cell clone?

11 November 2013 3,234 7 View