He Zhou

2 Questions 6 Answers 0 Followers

Questions related from He Zhou

How to troubleshooting a failed heterologous expression of a human protein?

I purchased the human clone of this gene and sub cloned it into a Xenopus oocyte plasmid (it contains Xenopus oocyte beta globin to enhance the expression in Xenopus oocyte) with a polyA site. But...

09 June 2023 6,992 5 View

When running DNA electrophoresis, why add EtBr to the gel instead of the DNA sample or the DNA dye?

Is there a specific reason for adding EtBr to the gel instead of DNA sample/dye mixture? I use a RNA dye containing EtBr when running RNA gel so I don't add EtBr to the agarose. Just curious if I...

07 June 2023 2,813 4 View