Hazrat Bilal

4 Questions 8 Answers 0 Followers

Questions related from Hazrat Bilal

How i can confirm my plasmid from single RE and double RE digestion?

I am trying to insert my gene in PcDNA3.0. after plasmid construction I confirm it via sequencing which were all right but when I try to express my gene in HeLa and 7702 cell line it were not...

06 June 2019 2,051 4 View

Why my DNA is shear on gell? can i use it for metagenomic study?

i am trying to extract DNA from soil via DNeasy powerSoil extraction kit. by onedrop spectrophotometry the DNA quantity is 104 A260/280 is 1.8 and A260/230 is 1.6 but on gell the DNA is sheared...

10 October 2018 8,447 4 View

Is it possible to express a gene in a vector?, detected in metagenomics?

if I analyze a soil sample by whole genome shotgun and detect some unknown gene. can I express them by experiment to find its product and so on.

10 October 2018 4,178 4 View

Can we determined the product size of amplicon by multiAlin software?

multiAlin software for different fasta sequence of same Gene with selected primers give us different size and site. For my gene.. we check out different fasts sequence.. it's give us size of 309.....

07 July 2017 1,799 0 View