Hazirah Azizan

2 Questions 4 Answers 0 Followers

Questions related from Hazirah Azizan

PCR gradient temperature ?

I have a question regarding how do we know what temperature should we choose for the gradient in the pcr machine. For example, I have primer with average temperature= 57.4. I need to use 6...

02 January 2024 5,822 1 View

Why the band of the PCR become double?

Hi, I have a question regarding my PCR products. Okay so i did some primer optimization for primer 1 using gradient temperature starting 55-60’C and i chose temperature 56.6’C as my temperature as...

01 January 1970 1,635 8 View