Hayley Barnes

2 Questions 2 Answers 0 Followers

Questions related from Hayley Barnes

QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

09 February 2021 533 3 View

How to isolate RNA in a way that is representative of an entire brain (fish)?

Hi there, I'm looking to compare the expression of a particular gene in fish brains across three developmental stages. My aim (as first step) is to use the entire brain for RNA isolation steps....

03 September 2020 7,285 3 View