I used the same standard concentration but with a different column type in HPLC. The area is much bigger now. Is this normal or I did something wrong?

The previous column was C8 column. Now I am using Chiral AMY1 column. The same HPLC device. The same lamp and wavelength. I read before that changing flow rate affects peak area. Are there any...

13 October 2023 7,809 6 View