Show a distinct peak while injecting HPLC Water as a sample and no peak running blank in Reverse phase HPLC using C18 column?

Wash column 100% MeOH and 100% ACN until to get baseline stable. After that equilibrate with mobile phase ( 97% 0.1% Trifluoroacetic acid (TFA)+3% ACN) for 30 minutes. After that run blank. It...

30 January 2023 8,865 3 View