Hasan Koyun

2 Questions 1 Answers 0 Followers

Questions related from Hasan Koyun

Why do I detect a monomorphism after a treatment of PCR-RFLP?

Hi everyone, I have got this question for you. I have been working on a sheep lactoalbumin (LALBA) gene to seek for polymorphism via PCR-RFLP. My sequence has been digested by several enzymes (by...

04 April 2015 2,002 5 View

Why were 65 sheep detected as monomorphic for the Lactoglobulin (LALBA) gene using PCR-RFLP method?

Primers were designed for LALBA gene based on coding squence (Exon 5) and for RFLP PCR product was cut EcoRI. We have only seen two digested DNA bands on agaraose gel for 65 animals upon genotyping?

12 December 2014 6,536 2 View