Harvijay Singh

3 Questions 10 Answers 0 Followers

Questions related from Harvijay Singh

Why a plasmid which transformed very well in DH10B cells, are not being transformed in BL21or Rosetta strains?

Hi all, I have set of constructs in pE-SUMO and pET49b vector, with all of which I can do CaCl2 mediated transformation of DH10B or DH5alpha cells, but neither of them are being transformed in...

06 December 2019 9,700 0 View

What could be the reason & possible solution of bacterial culture ceasing to grow after IPTG induction ?

Hi,,, Greetings of the day!I have been working on a His-tagged viral proteins (expression and purification in E coli. using pET28c vector) from last four year. And this protein have been kept on...

07 September 2017 9,378 3 View

What could be the potential reason of difference in the behaviour of a protein that it is a monomer in solution but a tetramer in crystal structure ?

Hi... I am working on a viral protein. When I purify it in the normal condition (Tris buffer, pH-7.5, NaCl & Glycerol, Temp- 4C), it is a monomer. But, as its crystal structure reveals, upon...

16 July 2017 8,554 3 View