Harvijay Singh

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Questions related from Harvijay Singh

Why a plasmid which transformed very well in DH10B cells, are not being transformed in BL21or Rosetta strains?

Hi all, I have set of constructs in pE-SUMO and pET49b vector, with all of which I can do CaCl2 mediated transformation of DH10B or DH5alpha cells, but neither of them are being transformed in...

06 December 2019 9,655 0 View

What could be the reason & possible solution of bacterial culture ceasing to grow after IPTG induction ?

Hi,,, Greetings of the day!I have been working on a His-tagged viral proteins (expression and purification in E coli. using pET28c vector) from last four year. And this protein have been kept on...

07 September 2017 9,321 3 View