Harshita Tiwari

2 Questions 1 Answers 0 Followers

Questions related from Harshita Tiwari

Why I am not getting colonies after transformation of plasmid with ligated insert?

I want to clone a 67 bp insert into the prmc2 vector. I have annealed primers with RE site overhangs. I got false colonies without dephosphorylation of the vector and no colonies after...

24 January 2025 5,257 7 View

How can I find UTR region sequence of a particular bacteria mRNA with the help of bioinformatics?

I want to find the UTR sequence of mRNA sequence of bacteria protein. Can anyone suggest a insilico process for that

23 November 2023 3,778 3 View