Harry Jenkins

4 Questions 3 Answers 0 Followers

Questions related from Harry Jenkins

Mouse CD4+ T-Cell Positive Selection, FACS, and anti-CD3/CD28 Stimulation?

Hello all, I have a couple of queries. I am not too experienced with these methods so advice would be appreciated. My current work requires the purification of CD4+ T Cells from mouse spleen and...

24 July 2019 8,242 5 View

Importance of light chain type on isotype control antibodies for flow cytometry?

I am putting together a panel for flow cytometry, for one particular antibody the only available isotype control has a different light chain to the test antibody. My test antibody is BV711-CD25...

11 June 2019 3,563 2 View

Optimising RT-qPCR for the detection of very low abundace IL-17A transcripts from human PBMC mRNA?

I am performing qPCR to quantify cytokine expression in human peripheral blood mononuclear cells. I have found it very difficult to get consistent results when assaying for IL-17A. Currently I am...

24 January 2019 8,496 1 View

Can DNase treatments to RNA preparations cause damage or loss to the mRNA?

When performing qPCR, I have noticed that cDNA made from RNA preparations treated with Turbo DNase (Invitrogen) prior to reverse transcription produce CT values that are shifted several cycles...

01 November 2018 9,846 5 View