Haroon Iqbal

4 Questions 19 Answers 0 Followers

Questions related from Haroon Iqbal

Antagonists for mannose receptor?

I am trying to verify the binding of a polysaccharide to the mannose receptor, any recommendations on a possible antagonist that I can use ? Many Thanks

07 January 2015 7,635 5 View

Why does the expression of MHC 1 decrease when lymphocytes are stimulated with LPS ?

I have noticed whilst using Beta 2 Microglobulin as a reference gene, the expression reduced significantly whilst doing PCR, can any one explain this? Cells were stimulated with LPS.

03 November 2014 3,588 3 View

In macrophage activation ( RAW264.7) why does the proliferation of macrophages stop when they are activated with LPS?

There is an increase in NO production and increase in phagocytosis (increased uptake of Neutral Red) but Alamar Blue assay shows a drop in cell numbers. Any explanation?

01 November 2014 4,811 7 View

What is the best way to precipitate proteins, from an extract, which also has polysaccharides?

I can´t use TCA as this will damage cells and I will be going on to test this fraction against cells.

20 July 2012 6,394 19 View