Harold Bell

4 Questions 3 Answers 0 Followers

Questions related from Harold Bell

Nuclear staining of Glucocorticoid Receptor antibody at baseline?

I am examining GR translocation in Jurkat cells upon treatment with dexamethasone(10^-6M). Slides are simple cytospins and not embedded. All papers on the subject suggest largely cytoplasmic...

09 November 2017 5,357 1 View

Does anyone know if rbc lysis buffer will cause activation of PBMCs during isolation?

We would like to examine cytokine release as well as MAPK phosphorylation in PBMCs and I would like to be sure the PBMCs will not be stressed and therefore activated by the RBC lyis step. Thanks...

31 May 2017 7,205 3 View

Can anyone recommend methanol vs formaldehyde for fixation of PBMC cytospins? And also for storage of the cytospins? Is long term storage possible?

I seem to find from searching google and such that it depends on what you will look at by IHC. Since we are not exactly sure at the moment, I'm looking for what would be the most universal...

03 May 2017 4,683 2 View

Hi - I am wondering how many human PMBCs are needed to have sufficient protein to perform a western blot- We plan on measuring phospho-p38?

PBMCs, western blot, p38, phosphorylation, total protein

17 March 2017 5,055 6 View