8 Questions 19 Answers 0 Followers
Questions related from Harir Mohamed
I have already immobilized mushroom tyrosinase onto Eupergic support have anyone a protocol or papers regarding how to check the ativity for the Immobilize enzyme onto the Eupergic carrier ? i...
14 June 2016 8,763 4 View
I tried to immobilized tyrosinase onto amberlite and without using Gluteraldyde Gluteraldyhde, and i have got 100 percent of bound (immoblize enzyme), aim the problem is there is no activity and i...
10 June 2016 2,345 8 View
I tried to immoblize tyrosinase enzyme onto amberlite beads using Gluteraldhyde to link the Amberlite with the Enzyme , and my question is why I didn t get any activity after activite the...
08 June 2016 8,043 3 View
Dear All, i have performed 16 S DNA molecular Identification of 7 actinobacteria isolates, after blasting , I have got a lot of streptomyces species that coresponding to my isolates at 100 per...
01 June 2016 5,203 3 View
Dear All, I have enzymatic crud extract from Actinobacteria , I d like to extract and purify Tyrosinase, and when I use L dopa i found a heigh activity and for 2,6 DMP substrat there were no...
19 May 2016 6,411 2 View
Dear all, I want to precipitate protein from supernatant of bacteria in culture and my question is whenever i do ammonium sulfate fraction i get floating pellet but, not getting settled pellet....
02 March 2016 5,041 7 View
Dear Researchers, I performed Tyrosinase purification from bacteria broth medium , but I have difficulty to purify this enzymes because of the presence of melanin. Does someone have any idea on...
21 January 2016 3,431 2 View
I want to purify tyrosinase enzyme from streptomyces bacteria after production in liquid medium and I d like to use tyrosine to check the activity ?
18 January 2016 5,424 3 View