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Questions related from Harinder Singh
If one wants to improve the enzyme activity by protein engineering, which kinetic parameter is crucial?
04 December 2015 8,868 11 View
I am sonicating DE3 cells for extracting my recombinant protein. I am getting my protein and things are working fine. But, I am curious to know about over or under sonication and overestimation...
18 September 2014 8,314 3 View
My extracted GC sample (in ethyl acetate) has some residual water in it, which I want to remove before GC injection. I will be using calcium sulphate, but I am not getting any clear protocol for...
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If its reading frame has a start codon and no stop codons in between, is there any chance that it will make a protein?
24 August 2012 7,969 4 View
I am using constant systems French press. There is lot of volume loss and frothing happening. I am working with bacterial cell suspension and volumes are around 1-2 ml. All kind of suggestions are...
13 August 2012 539 4 View
My lab has a regular set up for protein electrophoresis and blotting. I want to use the same for RNA blotting. Which recipe is the best and least time consuming to make my whole setup RNase free?...
22 June 2012 5,503 3 View
I have some intergenic small RNA candidates in prokaryotes which I want to validate.
05 June 2012 6,857 6 View