Harinder Singh

7 Questions 18 Answers 0 Followers

Questions related from Harinder Singh

If one wants to improve the enzyme activity by protein engineering, which kinetic parameter is crucial?

04 December 2015 8,866 11 View

What are the most suitable conditions for E. coli DE3 sonication?

I am sonicating DE3 cells for extracting my recombinant protein. I am getting my protein and things are working fine. But, I am curious to know about over or under sonication and overestimation...

18 September 2014 8,312 3 View

How do you remove residual water from GC samples?

My extracted GC sample (in ethyl acetate) has some residual water in it, which I want to remove before GC injection. I will be using calcium sulphate, but I am not getting any clear protocol for...

28 August 2014 4,770 20 View

What is the most important feature/parameter for considering a gene as a pseudogene?

If its reading frame has a start codon and no stop codons in between, is there any chance that it will make a protein?

24 August 2012 7,967 4 View

What is the trick to working with low volumes in a french press?

I am using constant systems French press. There is lot of volume loss and frothing happening. I am working with bacterial cell suspension and volumes are around 1-2 ml. All kind of suggestions are...

13 August 2012 534 4 View

Using western blot apparatus for northern blotting?

My lab has a regular set up for protein electrophoresis and blotting. I want to use the same for RNA blotting. Which recipe is the best and least time consuming to make my whole setup RNase free?...

22 June 2012 5,499 3 View

What methods are there other than northern blotting for small RNA validation in prokaryotes?

I have some intergenic small RNA candidates in prokaryotes which I want to validate.

05 June 2012 6,855 6 View