Regarding FRET: A GEF with YFP tag, though very well expressed gives less fluorescence. Do you have any ideas how to improve the fluorescence?

In single molecular FRET, a GEF protein with N-terminal YFP and C-terminal CFP gave nice YFP and slightly lower CFP signal. The same protein with the YFP tag on either N or C-terminal gave less...

08 December 2014 318 2 View