Can anyone help with a problem with wired band after EcoRI and XhoI digestion?

I was trying to insert the interested gene (2 kb) to the vector (6 kb). Firstly, I used EcoRI and XhoI to cut the plasmid, and treated with CIAP. Secondly, ligate the insert and vector by using T4...

24 November 2012 5,680 1 View