Haofei Liu

3 Questions 6 Answers 0 Followers

Questions related from Haofei Liu

How many cells in mouse mesenteric lymph nodes (MLN), and how many CD4 T cells in it?

22 March 2016 6,810 2 View

Is it normal that after electroporation, Bacillus subtilis was cultured with Recovery Media in shaker for 3 hours then became clear?

After electroporation, Bacillus subtilis was cultured with Recovery Media in shaker at 100rpm, 37°C for 3 hours. The Culture became clear, is it normal?Growth Medium: LB + 0.5M SorbitolETM: 0.5M...

01 November 2014 2,426 6 View

During the experiment, I found that the Bacillus subtilis cell storaged at 4 °C was dissolved.Is that phage contamination?

The bacillus subtilis was storaged at -70°C. After reticle on Culture plates, I cultured them in shaker at 200rpm, 37°C for 18h. Then the culture was placed at 4°C. One week later, when I want to...

12 October 2014 5,388 6 View