Hao Zhu

3 Questions 12 Answers 0 Followers

Questions related from Hao Zhu

How to improve the hibridization quality between RNA and DNA?

I am trying to use DNA probe to capture long RNA sequence. I heat the RNA to break the loop and add RNase inhibitor to protect it. However, the result always show RNA/DNA binding but denature...

03 March 2014 1,901 6 View

What is the attaching strategy for DNA oligo and Fe3O4?

I am going to attach my DNA oligo probe to either Fe3O4 nanoparticles or SiO2 coated Fe3O4 NPs. How should I modify my oligo? Which chemical groups give the best attachment and bio-safety property?

02 February 2014 4,054 2 View

Anyone knows which is the best reagent for DNA oligo denaturation at room temperature?

I am studying viral oligo detection. I need to seperate a dsDNA oligo at around 30 degree. Which chemical is the best to use? I heard that high concentration of salt might do that but I am not...

01 January 2014 1,012 19 View