Hao Zhang

6 Questions 4 Answers 0 Followers

Questions related from Hao Zhang

Is there any human cell line that have fair in situ growth capability yet have mild or none invasive migration probability?

Is there any human cell line that have fair in situ growth capability yet have mild or none invasive migration probability? Looking forward to your reply.

11 November 2016 7,226 1 View

Why is illumina sequencing index different from others?

"Illumina’s “indexing” system differs from other sample barcoding methods for high-throughput sequencing in that the barcodes (“indexes”) are placed within one of the adapters rather than...

08 August 2016 8,687 5 View

How to efficiently normalize a next generation sequence data?

Is there any application on mac recommended for NGS data analysis? Including normalization, differential expression and other common practice. R language package or video practice are also...

08 August 2016 7,920 5 View

How to use control gRNAs in Cas9 system?

recently I came across this paper: Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis It says in 67,405 single-guide RNAs (sgRNAs) there are 1000 control gRNAs. And I want...

08 August 2016 3,249 0 View

Why MISSION shRNA library cell gDNA retrieve PCR product is larger than predicted?

This is a mission shRNA library retrieve PCR process. The given primer in the kit called LAP1 and LAP2 product is 342bp, which is too large to proceed the PE150 illumina sequencing. So I...

11 November 2015 1,694 0 View

Why PCR using dUTP synthesized primer doesn't work?

Recently I used dUTP synthesized R6-Tag as R-Primer and a promoter specific primer as F-primer for round 1, and Tag with another promoter specific primer for round 2. UDG 37 degree for 30min. Yet...

05 May 2015 3,899 1 View