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Questions related from Hao Chen
I am recently conducting TUNEL on (perfused) mouse brain cryosection and so frustrated by its edge effect in DNase-treated positive control (The attached photo is a closeup of edge area). Not as...
03 July 2015 8,321 13 View
To distinguish protein splicing isoform seems to be quite difficult by using mass spectrometry, because of the nearly identical sequence of them. And after fragmentation in mass spectrometry,...
13 June 2015 2,920 7 View
I am preparing grip strength test for the first time. According to previous papers, people give three or five trials for each mouse and then these results are averaged to one. Seemingly these...
23 May 2015 4,532 5 View
Two years ago, Zhang et al. found mitophagy or autophagy for mitochondria is protective in the reperfusion phase, while deleterious in the earlier ischemia phase. But at that time, seemingly they...
25 April 2015 6,558 2 View
Gallic acid (GA) and Chlorogenic acid (CGA) are two types of secondary metabolites with good antioxidative activity. Recently I wonder, on mouse neuron culture, how glutamate-induced neuronal...
03 March 2015 2,841 1 View
We conducted primary neuron culture from mice embryo cortex recently and met this weird phenomenon: a suspending cloud of sticky white indigestible remnant after typsin incubation. Similar case...
05 February 2015 3,829 10 View
Five days ago we extracted cortex neurons from mouse embryos and today many cell clusters have arisen. A photo of them is attached below. And we noticed when there was no coverslip in wells, less...
04 February 2015 9,623 12 View
According to JAX, it is not recommended to use male 3xTg mice as tested subjects because of the report that male transgenic mice may not exhibit the phenotypic traits originally described. But for...
20 October 2014 5,775 6 View
