Hansong Chae

3 Questions 2 Answers 0 Followers

Questions related from Hansong Chae

Why my ATAC-seq protocol did't work?

I performed ATAC-seq with Hela cell but it didn't work. qPCR graphs were good to calculate the number of additional PCR cycles but, when i assessed the quality of purified libraries with...

30 March 2021 6,511 1 View

Is it possible to use the only shRNA vector when I perform shRNA transfection?

I want to do a shRNA transfection experiment, but there are too many shRNAs to be tested. So, except for virus particle vectors, i wanna add only shRNA vector using...

08 September 2020 450 3 View

What is the problem with my siRNA transfection?

I tried a pool of 3 siRNA transfection in Hela cells using RNAiMAX but all of of them failed. 100nM conc siRNA and opti-mem were used at 40~50% cell confluency and then 6 hour later, added DMEM...

03 September 2020 9,188 4 View