Hannah Kozl

4 Questions 5 Answers 0 Followers

Questions related from Hannah Kozl

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

17 February 2021 5,288 2 View

Is there a way to detect mRNA directly from samples (without purifying)?

I'm interested in detecting mRNA transcripts from urine but I have not worked with mRNA before. Is there a way to detect mRNA directly (i.e. boiling) from the sample without using silica...

10 July 2020 939 0 View

How do you use Differential Scanning Calorimetry (DSC) for DNA melting temperature?

I am trying to use a DSC to determine precise melting temperature of my DNA strands. I am using a TA instruments Q2000, and I am using Tzero pans/lids which I crimp together. Does it matter which...

28 April 2019 2,150 5 View

How do you use Differential Scanning Calorimetry (DSC) for DNA melting temperature?

I am trying to use a DSC to determine precise melting temperature of my DNA strands. I am using a TA instruments Q2000, and I am using Tzero pans/lids which I crimp together. Does it matter which...

04 April 2019 2,307 6 View